Middelburg is located in Bhisho city, South Africa at the -31.4929, 25.0063 coordinates. Distance from Middelburg to other cities. are listed below, click on the city name to find distance between. Distance from Middelburg to Port Elizabeth 275 km, to East London 321 km, to Uitenhage 254 km. The green marker indicates the location of Middelburg.